AppliChrom® GPC & SEC columns for biopolymers and aqueous polymers

•     Optimally suited for aqueous SEC separations
•     Aqueous and highly hydrophilic polymeric base material
•     Polymethacrylate resin, cross-linked with polyhydroxylated ether (surface containes some residual carboxyl functional groups)
•     Stable between pH 2 - 12.5
  
•     Pressure stable for 30-80 bar
•     Temperature stability between 10-85 °C
•     Low column bleed for low detector noise
•     Large pore volume for optimal resolution
•     Individual pore sizes for individual molecular weight ranges
•     Multi-pore technology for a wide range of molecular sizes
•     Increased lifetime of SEC columns through combination of proprietary particle and packing technology
•     Recommended eluents: H₂O, buffers
•     Application examples: Water soluble (bio)polymers up to 40 000 kDa like proteins, oligonucleotides, virus, heparin, polyethylene oxide (PEO), polyethylene glycol (PEG), pullulan, maltodextrin, inulin, pectine, polycarboxylate ethers, gelatine, dextranes, polyacrylamide (PAM), PEGylated proteins acrylate dispersions
  

AppliChrom® cation-exchange columns for oligo- and polysaccharides

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all.

But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist. But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy?

I know that I´ll never stand a chance of appearing in the Economist. But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

AppliChrom® Polymer based reversed phase (RP) columns

•     Rigid, spherical styrenedivinylbenzene copolymer with 
•     Particle sizes between 3 to 30 μm
•     Pore size of 100 Å
•     Reversed Phase (RP) separation with orthogonal selectivity to silica based columns
•     Improved chemical stability
•     Stable between pH 1.5–14
•     Temperature stability 10–90 °C
•     Stable towards pressures up to 150 bar
•     Low backpressure with high plate counts, ideal for preparative applications
•     Low column bleed for low detector noise
•     Up and down scaling ensured beteween analytical RP-HPLC and preparative process chromatography
•     Recommended eluents:  H₂O/ACN or MeOH with up to 1 % TFA, FA or NaOH additive
  
•     Application examples: Polar peptides, proteins or vitamins, hydrophobic amines
  

AppliChrom® SEC columns for water soluble (bio)polymers

•     Multi hydrophilic layered SEC media for highest efficiency and low interaction
  
•     Available seperation ranges from small molecules up to 5 500 kDa
  
•     Temperature stability 10-90 °C
•     Stable between pH 2–10
•     Pressure stability up to 200 bar
•     Simple adaptation from HPLC to SEC with stainless steel instruments
•     Versatile tool for accurate protein molecular weight or size determination
•     Low column bleed for low detector noise
•     Increased lifetime of SEC columns through combination of proprietary particle and packing technology
•     Recommended eluents: H₂O, buffers
•     Application examples: Water soluble proteins, humic substances
  

AppliChrom® SEC columns for proteins

•     Pure silica-based SEC media as alternative selectivity to VivoSep SEC columns
  
•     Designed to seperate proteins only by molecular size in the range of 4–400 kDa
  
•     Long term pH stability beween pH 2–7.5
•     Available pore sizes: 100, 200 or 300 Å
•     The addition of of organic modifiers is possible (e.g. 10–30 % MeOH or ACN)
•     Optional cleaning procedures with DMSO or THF extend the lifetime
•     Recommended eluentes: H₂O, buffers, optional with modifiers
  
•     Application examples: proteins, oligonucleotides, DNA, RNA, dextrane, pullulan, polysaccharides, pektins, Immunoglobulins, Caseine, polyethylene oxide (PEO), polyethylene glycol (PEG)

AppliChrom® GPC columns for GPC analysis of organic molecules with THF, toluene, chloroform

•     Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution
•     high pressure stability up to 150 bar
•     Temperature stabel between 10-145 °C
•     GPC range from 100 up to 50 000 000 Da
•     Low column bleed for low detector noise
•     High purity to eliminate adsorptive effects and combine pure GPC with long lifetime
•     Large calibration ranges for several decades due to the unique process of BPT technology
•     Range of individual porosities that can be used individually or in combination
•     Optimized for 1 ml/min flow rate for 8 mm ID columns
•     High level of reproducebility and peak precision due to the advanced packing procedure
•     Recommended eluents: THF, Toluene, CHCl₃, dichlorbenzene, hexane/EtOAc (10:90, V:V)
  
•     Application examples: Hydrophobic oligomers or polymers like PVC, PMMA, polystyrene, epoxy resins, polyesters, bisphenol A resins
  

AppliChrom GPC columns for organic GPC analyses (DMAc or DMF)

•     Organic polymer base material with high porosity for high resolution
•     Wide range of porosities and particle sizes
•     Pressure stability up to 150 bar
•     Stable between 35-145 °C
  
•     Excellent reproducibility and resolution due to the advanced packing procedure
•     High purity to eliminate adsorptive effects and combine pure GPC with long lifetime
•     GPC range from 100-1,000,000 Da
•     Optimal for 0.5 ml/min flow rates with 8 mm ID colums
•     Recommended eluents: DMAc, DMF, NMP, LiBr additive
  
•     Application examples: Slightly polar analytes like PMMA, polyacryl nitril (PAN), acrylnitrile butadiene styrene (ABS), cellulose, and all DMF and DMAc soluble polymers
  

 AppliChrom® ABOA DMSO-PHIL optimized for DMSO-GPC applications

•     Interaction-free pure GPC base material
  
•     Temperature stabel between 40-90 °C (145 °C) depending on application
  
•        Simple, reliable and robust GPC & SEC calibration from Dextrans, Pullulans et al.
•     Low column bleed for low detector gain for improved refractive index, light scattering or viscosity detection
•     12µ particle technology for low back pressure
•     Tolerates traces of water and NaNO₃ additve
  
•     Large volume and optimized mass transfer for polymers with optimized resolution
•     Low cost due to long column lifetime - result of the combination of optimized particle and packing technology
•     Recommended eluents: DMSO, 0.075 M NaNO₃
•     Application examples: Polar, not water soluble oligomers or polymers like polysaccharides, lignin, amylose, starch, humic acids, polyvenyl pyridin, PNIPA, polyvinyl alcohol (PVA), melamin-urea-formaldehyd resin (MUF) or urea-formaldehyd resin (UF)

AppliChrom® GPC columns for separations in HFIP

•    Broad pore technology for GPC for analytes with challenging solution behavior
•    Separation range beween 0.1–50 000 kDa
•    Range of individual porosities that can be used individually or in combination
•    Temperature stable between 35–145 °C
•    Long column lifetime - result of the combination of optimized particle and packing technology
•    Low column bleed for low detector noise
•    Excellent reproducibility and resolution due to the advanced packing procedure
•    Calibration standard: PMMA or PEO  
  
•    Recommended eluents: Hexafluoroisopropanol (HFIP), 0.1 % KTFa as modifier
•    Application examples: Crystalline highperformance polymers like polyamides (PA), polyethylen terepthalate (PET), polybutylen terepthalate (PBT), polylactic acid (PLA), polyamid 6 (PA6), polycaprolactam, paraformaldehyde, polyoxymethylene (POM), polyacetal, several polyoxazolines

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist.

But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist.

But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist.

But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist.

But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.

Headline headline head

I am a dummy copy. And I´ve been a dummy copy since my birth. It took me a long time to realize what it means to be a dummy copy: you make no sense. You stand out now and then by being completely out of context. Often, you aren´t even read at all. But does that make me a bad copy? I know that I´ll never stand a chance of appearing in the Economist.

But does that make me any less important? I´m a dummy! But I enjoy being a copy. And should you now decide to carry on reading me to the end, I´ll have managed to achieve something most „normal“ copies don´t achieve.